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Consider the following statements:
I. Recombinant DNA technology is popularly known as genetic engineering is a stream of biotechnology which deals with the manipulation of genetic materials by man invitro
II. pBR322 is the first artificial cloning vector developed in 1977 by Boliver and Rodriguez from E.coli plasmid
III. Restriction enzymes belongs to a class of enzymes called nucleases.Choose the correct option regarding above statements
The process of recombinant DNA technology has the following steps
I. amplication of the gene
II. Insertion of recombinant DNA into the host cells
III. Cutting of DNA at specific location using restriction enzyme .
IV. Isolation of genetic material (DNA) Pick out the correct sequence of step for recombinant DNA technology.