Biotechnology and its Applications - Online Test
Q1. Mobile genetic elements that replicate an RNA intermediate are called as
Answer : Option A
Explaination / Solution:
Mobile genetic elements that replicate an RNA intermediate are called transposons. It is a small piece of DNA that inserts itself into another place in the genome.
Q2. Alpha lactalbumin present in milk is
Answer : Option A
Explaination / Solution:
The milk produced by transgenic cow contain a protein called alpha lactaalbumin. The milk containing this protein is more suitable for babies as compare to normal cow’s milk.
Q3. Gene transfer in biotechnology can be done by
Answer : Option C
Explaination / Solution:
Gene transfer in biotechnology can be done by number of methods. Microinjection is the use of a glass micropipette to inject a liquid substance at a microscopic or borderline macroscopic level. The target is often a living cell but may also include intercellular space.
Microinjection is one of the best methods to transfer desired gene into suitable host.
Q4. Plasmid used to produce humulin is
Answer : Option B
Explaination / Solution:
Plasmid are extra nuclear genetic materials present in bacterial humulin is produced by using plasmid Pbr322. It is an E.coli plasmid vector containing the origin of replication.
Q5. Expand GEAC
Answer : Option A
Explaination / Solution:
The Genetic Engineering Approval Committee (GEAC) is the apex body constituted in the Ministry of Environment and Forests under 'Rules for Manufacture, Use, Import, Export and Storage of Hazardous Microorganisms/Genetically Engineered Organisms or Cells 1989', under the Environment Protection Act, 1986.
Q6. alpha endotoxins are formed by
Answer : Option B
Explaination / Solution:
Alpha endotoxins are formed by sporulation. Alpha endotoxins are known to be frequent contaminants in plasmid DNA extracted from bacteria.
Q7. DNA is denatured to produce single strand by
Answer : Option C
Explaination / Solution:
Deoxyribose nucleic acid is denatured to produce single stranded by using alkaline solution. DNA is acidic in nature, in alkaline solution the hydrogen bonds between the nitrogenous bases is broken to separate two strands.
Q8. Radioactive probe can be detected by
Answer : Option A
Explaination / Solution:
Radioactive probes are single stranded DNA or RNA having radioactive components. This probe can be detected in the individual by using autoradiography technique.
Q9. Rate of fluorescence in a titer plate in ELISA is
Answer : Option C
Explaination / Solution:
The rate of fluorescence in a titer plate in ELISA is directly proportional to antigens present in the sample. This indicates presence of pathogen in the sample also.
Q10. Viral infection can be treated by
Answer : Option D
Explaination / Solution:
Interferons can be sued to treat viral infection as interferon are proteins made and released by host cells in response to presence of pathogen as viruses, bacteria or tumor cells.
